Plantactinospora mayteni gen. nov., sp. nov., a member of the family Micromonosporaceae

Abstract

A novel Gram-positive, aerobic, spore-forming, endophytic actinomycete, designated strain YIM 61359(T), was isolated from the roots of Maytenus austroyunnanensis plants collected from tropical rainforest in Xishuangbanna, Yunnan Province, south-west China. The strain formed single or cluster spores with smooth surfaces from substrate mycelia. The strain contained meso-diaminopimelic acid in the cell wall and arabinose, xylose, galactose and glucose in whole-cell hydrolysates. The acyl type of the cell-wall polysaccharides was glycolyl. MK-10(H(6)), MK-10(H(8)) and MK-10(H(4)) were the predominant menaquinones. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and several unknown phospholipids. The major fatty acids were iso-C(15 : 0), anteiso-C(15 : 0), C(17 : 0), anteiso-C(17 : 0) and iso-C(16 : 0). The DNA G+C content of strain YIM 61359(T) was 69.7 mol%. These chemotaxonomic data indicated that the strain belongs to the family Micromonosporaceae. Phylogenetic analysis based on 16S rRNA gene sequences also suggested that strain YIM 61359(T) fell within the family Micromonosporaceae. On the basis of morphological and chemotaxonomic data, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain YIM 61359(T) is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Plantactinospora mayteni gen. nov., sp. nov. is proposed. The type strain of Plantactinospora mayteni is YIM 61359(T) (=CCTCC AA 208022(T)=DSM 45238(T)).