Plant-wax alkanes as seasonal markers of red deer dietary components

Abstract

n-Alkanes are long-chain hydrocarbons commonly occurring in plant cuticles that can be recovered in herbivore faeces. Differences among plant species in their content of cuticular wax n-alkanes can be exploited to estimate diet composition of herbivores. n-Alkanes have been used mainly in domesticated herbivores feeding on relatively simple diets over short-term periods. Extending the method to wild herbivores feeding on seasonal complex diets is possible provided that n-alkanes act as effective dietary component markers in different seasons. The n-alkane content of browse species and herbage layer and of faeces of red deer (Cervus elaphus L., 1758) males and females in a region with a Mediterranean climate is described. Information on the n-alkane content of plant species was related to that of faeces to estimate the diet composition of red deer. Plant species had distinct n-alkane contents, some of which varied seasonally. The n-alkane content of faeces also varied seasonally and between red deer sexes. Both red deer males and females had relatively high proportion of browse in their diets during summer and of herbage layer in spring, as shown by other studies in Mediterranean environments.