Plant Volatiles Mediate Host Selection of Sitodiplosis mosellana (Diptera: Cecidomyiidae) among Wheat Varieties.

Abstract

Sitodiplosis mosellana is a major wheat pest that oviposits on spikes, and resistant wheat varieties have been released. However, wheat spike volatiles mediating S. mosellana host selection or resistance are largely unknown. In this study, we found that the highly susceptible wheat varieties Xinong 822, Xinong 88, and Xiaoyan 22 were preferred for S. mosellana oviposition, and their spike volatiles were more attractive to females compared to the resistant varieties Kenong 1006, Shanmai 139, and Jinmai 47. Importantly, we found five odor components evoking obvious concentration-dependent electroantennogram (EAG) and behavioral response. Notably, 3-hexanol, cis-3-hexenylacetate, and hexyl acetate strongly attracted females, whereas ocimene, a dominant component of three resistant varieties, and α-farnesene, absent in Xinong 88, repelled females. Significant attraction was also observed in a synthetic blend mimicking Xinong 822 volatiles. These results suggest that these wheat volatiles are involved in host selection of S. mosellana and provide a basis for development of semichemical-based pest management.