Plant virus uncoating as a result of virus-cell wall interactions

Abstract

The fate of tobacco rattle virus (TRV) particles was studied after leaf panel infiltration. It was found that end-on virus attachment to cell walls of hosts ( Nicotiana tobacum L. var. Xanthi-nc) and nonhosts ( Zea mays L.) occurred, and that a virus degradative phase commenced immediately after attachment. Length of TRV particles changed drastically following infiltration. The normal length of the particles became smaller than that of the particle carrying the coat protein gene (108 nm). Five days after infiltration, no particles could be detected on the walls of cells bordering intercellular spaces. Virus attachment and degradation was thus shown to be nonspecific. Autoradiographic studies showed that iodinated viral coat protein or a breakdown product of this protein ( 125I-label in coat protein of complete virus) and not virus particles are transported to the vascular bundle after infiltration. Tobacco mosaic virus (TMV) and TRV were infiltrated into their respective local lesion hosts. There was an initial time interval early after the infiltration during which wounding (pin pricking) the infiltrated leaf panels resulted in local lesion formation. The results are discussed with regard to the phenomenon of viral genome release in the plant virus infection process.