Abstract
Plants have been studied as bioreactors for over 30 years. Their advantages over other expression methods arouse interest in this heterologous protein expression system. Over the years, several methods of plant transformation were developed, and among them, the transient expression system - that occurs temporarily and without integration into the host genome. The use of viral vectors for this type of expression stands out, and the viral genome can be “full-length” or “deconstructed”. Some genome modifications in the “deconstructed” form help searching for efficient protein expression, and several types of viruses are studied to develop these vectors. This review reports different types of viral vectors, especially those particularly suitable for the development of effective vectors of fast and high yield recombinant protein production, based on Tobamoviruses, Potexviruses, Geminiviruses and Comoviruses.
Highlights
The idea of using plants to express proteins emerged during the 1980s (FRANKEN; TEUSCHEL; HAIN, 1997), and several studies using transgenic plants for the production of antibodies (HIATT; CAFFFERKEY; BOWDISH, 1997), hormones (BARTA et al, 1986), and vaccines (THANAVALA et al, 1995) were developed
The results indicated that the transient expression in tobacco plants is a promising way to produce a cheap and effective vaccine against HPV16
tobacco mosaic virus (TMV) was certainly the most explored Tobamovirus for the production of viral vectors, but other researchers, such as Liu and Kearney (2010) developed a vector-based on another tobamovirus - the Sun Hemp Mosaic Virus (SHMV) (Figure 2C) - that infects legumes and incorporates some of the attributes of the Icon and TRBO strategies
Summary
The idea of using plants to express proteins emerged during the 1980s (FRANKEN; TEUSCHEL; HAIN, 1997), and several studies using transgenic plants for the production of antibodies (HIATT; CAFFFERKEY; BOWDISH, 1997), hormones (BARTA et al, 1986), and vaccines (THANAVALA et al, 1995) were developed. When introduced into host plant cells, viruses are designed to contain a gene of interest replicate, allowing the expression of high levels of heterologous proteins in infected plants (LINDBO, 2007; HEFFERON, 2014; IBRAHIN; ODON; KORMELINK, 2019) This method offers many advantages over the transgenic system, including its compatibility with various plant species, reduced protein production time, and high profitability (HEFFERON, 2019). The advantages of viral vectors missing the CP are the lower risk of escaping to the environment and maximization of protein production efficiency This is due to their inability to systemically infect plants and of being transmitted by insects or other types of natural vectors, as well as the low metabolic energy directed to the expression of CP, abundant in the wild-type virus infected-cells (LINDBO, 2007). Some studies demonstrate that viral vectors for protein expression systems can already be considered consolidated, attractive, and competitive processes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
