Abstract
Rabies, a serious health hazard, affects central nervous system of mammals and is caused by rabies virus. The viral glycoprotein forms the external surface of virus and is the most antigenic protein. Hence the rabies glycoprotein is an ideal candidate for use in the construction of a subunit marked vaccine. With the idea to substitute the total inactivated virus with G protein in the production of vaccine, the study was undertaken to clone the full-length glycoprotein gene under the control of various constitutive and expression promoters for its expression in bacterial background and its further cloning for plant transformation.
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