Abstract
Plant Taste Affects Diet Selection by Plateau Zokors (Eospalax baileyi)
Highlights
A sequence was treated with PvuII restriction enzyme
We address the issue of a possible relationship between breast cancer and single nucleotide polymorphism (SNPs) in estrogen receptor gene (ESR1) in patients of district Bannu, KP, Pakistan
Large numbers of estrogen-related genes have been reported with single nucleotide polymorphisms (SNPs) as the probable risk factors of breast cancer in the women already have gone through menopause (Yager, 2000)
Summary
Blood samples of clinically diagnosed 18 breast cancer patients and 12 normal individuals were collected from district Bannu. All the individuals belong to Pashtun ethnic group (Table I) and were molecularly characterized for SNPs in ESR1 gene. Detailed information of each patient was recorded on study-designed pro forma. The study was approved by ethical committee of Gomal University, D. DNA was extracted by salting out method (Miller et al, 1998). Four ml of blood was washed with T.E (10 mM Tris HCl pH 8.0, 2 mM EDTA) after thawing. The pellet was resuspended in 4.5 ml buffer containing 10
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