Abstract
Immature embryos from Zea mays L. inbred line B73 were cultured on Murashige and Skoog (MS) medium containing 0.5 mg/1 2,4-dicholorophenoxyacetic acid (2,4-D) and 12% (w/v) sucrose. A compact white callus proliferated from the scutella. When this tissue was transfered to equivalent medium containing 2% (w/v) sucrose, a green organogenic tissue was selected from the compact callus. After over 2 years in culture, this tissue was still capable of regenerating plants. A sector of this tissue spontaneously became embryogenic. Embryogenic callus can be distinguished by its friability, rapid growth rate and its mucilaginous texture. Embryogenic callus and suspensions have been maintained for approx. 2 years. Plants from organogenic tissue and embryogenic callus have been grown to maturity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
