Abstract
Micropropagation of Sumatra Benzoin is potential to provide good-quality seed for future preservation of the forest and improve the incense sap production. The production of Styrax plants is currently limited by the availability of seed. This research demonstrated the micropropagation of Sumatra Benzoin (Styrax benzoin Dryander), producing good-quality saplings that could be used for obtaining nontimber forest products. Elite mother plant was selected and used as a source of explants. Identification of healthy trees was carried out based on the phenotype criteria, and the selection of a mother plant was performed through information on the quality and quantity production of incense sap. Micropropagation started from callus induction in young leaves followed by subculture to obtain regeneration of shoots and roots. The combination of NAA and BAP in the culture media greatly affected the growth and development of callus, shoots, and roots. The use of 3 mg/L NAA and 3 mg/L NAA rendered the heaviest calli. Shoots were regenerated with 0.5 mg/L NAA and 3.0 mg/L BAP, and the highest growth of roots was obtained by using of 3.0 mg/ NAA without BAP. This research reports the first in vitro propagation technique for Styrax benzoin. Further research is underway to obtain very good-quality plant saplings to be used for forest conservation and to increase the production of incense sap as a nontimber forest commodity.
Highlights
Indonesia is a tropical region with big forest areas that are sources of global oxygen
Shoots, and seed embryos were taken from the best-quality mother plants. e explants of young leaves that just grew on the branches of the plant produced the highest callus weight with high growth intensity with minimal fungal contamination
Callus regeneration occurred after six weeks, followed by shoot formation after 12 weeks (Figure 1(c)) and root shoots (Figure 1(d)). e variation in the combination of the concentration of growth regulators affects the intensity of growth and development of the Sumatra Benzoin plant
Summary
Indonesia is a tropical region with big forest areas that are sources of global oxygen. E benzoin trees grow well in the forest area in North Sumatera and produce a high-quality incense sap, known as frankincense. Is strategy makes it difficult to obtain uniform seedlings that produce goodquality incense sap, as some of the trees produce high quantity of gum resin while some other produce low International Journal of Forestry Research amount. It takes around 10 years for naturally growing plants to produce sap. E purpose of this study is to apply the micropropagation technique to selected Sumatra Benzoin (Styrax benzoin Dryander) plants, in an effort to produce good-quality saplings that produce frankincense in large quantities and with the same quality as the mother plant. Good-quality saplings are intended to be distributed for plantation of industrial forest and replantation of deforestated field in local area at Kabupaten Pakpak Bharat, North Sumatera, Indonesia
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