Abstract
A simple protocol has been developed for plant regeneration by somatic embryogenesis of Southern Adriatic iris (Iris pseudopallida Trinajstic), an endemic species of the Balkan Peninsula. Somatic embryogenesis was induced in zygotic embryo culture on media supplemented with 2,4-dichlorophenoxy acetic acid (2-10 mgL-1) as the sole plant growth regulator, where both embryogenic calli and somatic embryos were induced. Subsequent decrease of 2,4-D in the media promoted formation of somatic embryos. Developed somatic embryos germinated on medium without growth regulators. The regenerated plantlets had diploid chromosome number. Planted plantlets acclimatized very well under greenhouse and garden conditions.
Highlights
Dalm. & Montenegr., non L.) – belongs to the genus Iris, subgenus Iris, section Iris, and series Pallideae. This series consists of the cultivated species I. pallida, very important for the perfume industry and three indigenous and endemic Alpine-Dinaric species: I. cengualti Ambr., I. illirica Tomm., and I. pseudopallida Trinajstić (Mitić et al, 2001)
Besides the Southern Adriatic iris, there is only one more species from the genus Iris which is endemic to the Balkan Peninsula, I. reichenbachii (Šilić, 1991)
Isolated zygotic embryos of I. pseudopallida were cultured under conditions of eight treatments for induction of morphogenesis in vitro (Table 2)
Summary
& Montenegr., non L.) – belongs to the genus Iris, subgenus Iris (bearded irises), section Iris (eupogons, the true bearded irises), and series Pallideae. This series consists of the cultivated species I. pallida, very important for the perfume industry and three indigenous and endemic Alpine-Dinaric species: I. cengualti Ambr. Besides the Southern Adriatic iris, there is only one more species from the genus Iris which is endemic to the Balkan Peninsula, I. reichenbachii (Šilić, 1991). Somatic embryogenesis of iris species was induced for the first time in zygotic embryo culture of Dutch iris (Reuther, 1975). 2,4-D alone was used for induction of somatic embryogenesis in zygotic embryo culture of Iris germanica (Reuther, 1975, 1977) and bulb-scale culture of I. hollandica
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