Plant Regeneration from Shoot Tips-derived Callus of Ginger (Zingiber officinale Rosc.)

Abstract

A protocol was described to regenerate plants from callus culture of ginger (Zingiber officinale Rosc.). Callus cultures were induced from ginger shoot tips inverted cultured on semi-solid MS medium supplemented with 2, 4-D (0.5-1.0 mg L -1 ) and BA (0.51.0 mg L -1 ). Maximum callus induction was obtained on MS medium supplemented with BA (0.5 mg L -1 ) either alone or with 2,4-D (0.5 – 1.0 mg L), while the highest callus weight was on MS medium supplemented with BA (0.5 mg L -1 ) and 2,4-D (0.5 mg L -1 ). Induced callus was tested for regeneration on MS medium supplemented with different concentrations of 2,4-D and BA and the best regeneration was observed on a medium containing 2.5 mg L -1 BA. The regenerated plants were rooted and successfully established in the field after few days of acclimatization.