Abstract
Protoplast isolation, culture and shoot regeneration from protoplast-derived calli were compared among different organs of Dianthus species under the same conditions. Leaves, petals and seedling hypocotyls of D. caryophyllus cv. Chabaud (a seed-propagated cultivar) and D. barbatus were used as protoplast sources. Protoplasts isolated from petals of these two species showed only low yield and low division frequency. On the other hand, division frequency of hypocotyl protoplasts was higher than that of leaf protoplasts, although, in D. caryophyllus, protoplast yield in hypocotyls was slightly lower than in leaves. In both species, relatively high frequency of shoot regeneration was obtained in hypocotyl- and petal-derived protoplasts, while only low or no shoot regeneration occurred in leaf-derived protoplasts.
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