Abstract
Summary Datisca glomerata is a perennial plant with a herbaceous annual habit, that forms nitrogen-fixing root nodules in association with the microsymbiont Frankia sp. (Actinomycetales). Datisca offers an important possibility as a model system to study genetics and gene expression in an actinorhizal root nodule symbiosis. Neither regeneration by in vitro culture nor genetic transformation of Datisca have been reported to date. As the first step, we studied plant regeneration from leaf tissue of Datisca. In preliminary experiments with 16 treatments, the best regeneration media was MS + BA, 10 μM + NAA, 0.5 μM + phloridzin, 10 mg/l. The best regeneration efficiency ranged from 26 % to 33.3 %.
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