Abstract
The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, it was observed that medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79.66 ± 0.44%) and Q117 (82.83 ± 1.69%). Addition of PEG 8000 at 2.5% to this medium had a profound impact on inducing somatic embryogenesis in Co 86032 (54.66 ± 1.76%) and Q117 (66.66 ± 2.60%) as compare to control (24.33 ± 1.76%) and (27.33 ± 2.73%), respectively. Cell suspension cultures were established by culturing embryogenic calli in liquid medium showed well established suspension cultures with fever cell aggregates. There was negligible cell division during initial 2 days of incubation and cell count increased rapidly between 2 and 8 days. Further incubation beyond 8 days resulted in a decrease in cell viability. Enhanced callus proliferation in Q117 while enhanced shoot regeneration in Co 86032 was observed from cell suspension culture. The clonal fidelity of in vitro regenerated plants was assessed by using RAPD and ISSR markers. Analysis of the ten RAPD markers indicated that 90.48 and 86.95% true-to-type regenerated plantlets in Co 86032 and Q117, respectively. However, in the ISSR markers, Co 86032 did not show any polymorphism and in the Q117, 92.18% true-to-type plantlets were found. These results confirmed that somaclonal variation occurs during the process of indirect organogenesis and RAPD and ISSR marker based molecular analysis is a suitable method for an early detection of variation in sugarcane.
Highlights
Sugarcane (Saccharum officinarum L.), is an important cash crop which contributes to around 70–80% of sugar production globally
The present work was performed with an objective to establish sugarcane (Co 86032 and Q117) suspension culture and subsequent regeneration of plantlets and study their genetic variation occurred during the process of regeneration using molecular markers (RAPD and ISSR)
The filter paper containing harvested cells was carefully inoculated in petri plates containing callus induction medium supplemented with polyethylene glycol 8000 (PEG) 8000 (2.5%) and incubated at
Summary
Sugarcane (Saccharum officinarum L.), is an important cash crop which contributes to around 70–80% of sugar production globally. The RAPD and ISSR analysis has been widely used to determine polymorphism in genomic DNA in sugarcane and several other plant systems due to numerous advantages like being a relatively fast, simple, cost-effective technique which requires a small quantity of DNA sample with no preliminary sequence information for primer design (Suprasanna et al 2006; Devarumath et al 2007; Lal et al 2008; Rizvi et al 2012; Dangi et al 2014; Kshirsagar et al 2015). The present work was performed with an objective to establish sugarcane (Co 86032 and Q117) suspension culture and subsequent regeneration of plantlets and study their genetic variation occurred during the process of regeneration using molecular markers (RAPD and ISSR)
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