Abstract
ABSTRACT Callus was induced from nodal explants of mature tillering plants of Jamrosa, a hybrid Cymbopogon (Cymbopogon nardus var. confertiflorus × Cymbopogon jwarancusa) on MS basal medium supplemented with 3 mg/l of 2,4-D and 0.5 mg/l of kinetin. Shoot regeneration was achieved by transferring the callus to basal medium supplemented with 0.5 mg/l NAA, 1 mg/l kinetin and 1 mg/l benzyladenine. Shoot multiplication was achieved using newly developed adventitious shoots from calli on MS medium supplemented with 1.0 mg/l of benzyladenine. Complete plantlets were obtained when the regenerated shoots were transferred to basal medium devoid of growth regulators. A high frequency of plant regeneration was recorded from both non-irradiated and γ-irradiated callus. Regeneration potential was enhanced by a relatively low level of gamma rays, 1 to 10 Gray, whereas doses above 10 Gray, though stimulating callus growth, suppressed shoot regeneration.
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