Abstract
An efficient regeneration and transformation system was developed for hybrid poplar populus davidiana Dode × P. bollena Lauche. Several factors, such as a competent regeneration protocol, antibiotics, bacterial concentration, infection time have been shown to affect transformation efficiency. It is the first report describing an efficient protocol for Vacuolar H + -ATPase c Subunit Gene from salt tolerant plant Puccinellia chinampoensis , which transformed into populus . Stable transgene integration was confirmed by the expression of GFP fusion proteins in leaves, stem and root for populus.
Highlights
Development of an efficient, competent plant regeneration system is essential for transformation
Populus davidiana Dode × P. bollena Lauche is the hybrid cultivar of poplar that using Populus bolleana as male parent and Populus davidiana Dode as female parent, which is planted on a significant scale in northeast of China due to its characteristics as graceful shape, cold resistance, fast growth, and suitability to develop transgenic
We developed a high efficient regeneration system for Populus davidiana Dode × P. bollena Lauche
Summary
Development of an efficient, competent plant regeneration system is essential for transformation. Different genotype of poplar have been used to perform the regeneration experiment [1,2,3]. The technology has been applied to various populus species for transformation effeiency [5,6,7], The systematic analysis of several factors to improve the transformation efficiency, including poplar genotype, Agrobacterium tumefaciens strain, bacterial concentration and so on [8,9,10]. Populus davidiana Dode × P. bollena Lauche is the hybrid cultivar of poplar that using Populus bolleana as male parent and Populus davidiana Dode as female parent, which is planted on a significant scale in northeast of China due to its characteristics as graceful shape, cold resistance, fast growth, and suitability to develop transgenic
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