Abstract
Ellagic acid, a commonly occurring plant phenol, was shown to be a potent in vitro inhibitor of GSH-transferase(s) activity. Other plant phenols such as ferrulic acid, caffeic acid and chlorogenic acid also showed a concentration dependent inhibition of GSH-transferase(s) activity. The I 50 values of ellagic acid, caffeic acid, chlorogenic acid and ferrulic acid were 8.3 × 10 −5M, 14.0 × 10 −5M, 20.0 × 10 −5M and 22.0 × 10 −5M respectively, suggesting that ellagic acid is the most potent inhibitor of all the four sutudied plant phenols. At 55 μM concentration of ellagic acid, a significant inhibition (35–47%) was observed on GSH-transferase activity towards CDNB, p-nitrobenzyl chloride and 1,2-epoxy-3-(p-nitrophenoxy)propane as substrates. Ellagic acid inhibited GSH-transferase(s) activity in a non-competitive manner with respect to CDNB while with respect to GSH it inhibited the enzyme activity in a competitive manner. Other phenolic compounds purpurogallin, quercetin, alizarin and monolactone also showed a concentration dependent inhibition of the enzyme activity with a I 50 of 0.8 × 10 −5M, 1.0 × 10 −5M, 8.0 × 10 −5M and 16.0 × 10 −5M respectively. These inhibitors of GSH-transferase(s) activity should be useful in studying the in vitro enzyme mediated reactions of exogenous and endogenous compounds.
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