Abstract
Paulownia witches’ broom (PaWB) caused by a phytoplasma, has caused extensive losses in the yields of paulownia timber and resulted in significant economic losses. However, the molecular mechanisms in Paulownia that underlie the phytoplasma stress are poorly characterized. In this study, we use an Illumina platform to sequence four small RNA libraries and four degradome sequencing libraries derived from healthy, PaWB-infected, and PaWB-infected 15 mg·L−1 and 30 mg·L−1 methyl methane sulfonate (MMS)-treated plants. In total, 125 conserved and 118 novel microRNAs (miRNAs) were identified and 33 miRNAs responsive to PaWB disease were discovered. Furthermore, 166 target genes for 18 PaWB disease-related miRNAs were obtained, and found to be involved in plant-pathogen interaction and plant hormone signal transduction metabolic pathways. Eleven miRNAs and target genes responsive to PaWB disease were examined by a quantitative real-time PCR approach. Our findings will contribute to studies on miRNAs and their targets in Paulownia, and provide new insights to further understand plant-phytoplasma interactions.
Highlights
Phytoplasma are specialized bacteria, generally called mycoplasma-like organisms, which belong to the Mollicutes class
The 24-nt small RNA (sRNA) were the most abundant, with approximately 27.17%, 20.69%, 13.76%, and 19.07%, present in the PTF, PTFI, PTFI15 and PTFI30 libraries, respectively, followed by the 21-nt sRNAs, which suggested that the 21- and 24-nt sRNAs may play important roles in Paulownia witches’ broom (PaWB) disease resistance in Paulownia
To understand the molecular mechanisms that operate between the PaWB disease-causing phytoplasma and its host, we characterized the sRNAs in four Paulownia libraries based on the Paulownia UniGene transcriptome information
Summary
Phytoplasma are specialized bacteria, generally called mycoplasma-like organisms, which belong to the Mollicutes class. They live in the phloem tissue of plants and are transmitted by insect vectors [1]. It has been reported that phytoplasmas are the pathogens associated with a large number of diseases in several hundred economically important plants [2, 3], and Paulownia plants are no exception [4, 5]. When Paulownia plants become infected, the phytoplasma can move systemically by the phloem sieve tube elements and mainly accumulate in developing leaves, flowers, and roots, where symptoms including.
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