Abstract
A survey of the plant parasitic nematodes associated with Centella asiatica (L.) Urban was conducted in different localities of Manipur. Twenty one species of plant parasitic nematodes belonging to 12 genera were found to occur. Tylenchorhynelms mashhoodi, Aphelenchus avenae and Helicotylenchus dihystera were predominant with absolute densities ranging from 338 - 498 per 500g soil. Basiria varians and T.mashhoodi were recorded with highest absolute frequency and absolute density respectively.
Highlights
The most prevalent species of plant parasitic nematodes associated with C. asiatica were T. mashhoodi, A. avenae and H. dihystera with absolute densities 498, 382,338 per 500g soil respectively
The frequency of occurrence of Coslenchus bisuxualis, C. tausifi, Criconemella oblongata, C. ornata, C. serrata and Hirschmanneilla oryzae was less as compared to other nematode species
Helicotylenchus abunaamai was most common nematode species associated with a number of medicinal plants grown in a Silviculture Farm at Kalinga in the district of Phulbani, Orissa (Routaray & Das 1982)
Summary
Seventy soil samples including a portion of rootsystem were collected from the rhizosphere of C. asiatica from different localities of Manipur. The nematodes were isolated from the soil using Cobbs (1918) sieving and decanting methods and Baermanns funnel technique (Thorne 1961). The nematodes collected were killed and fixed in warm formalin glacial acetic acid [F: A (4:1)]. The nematodes were identified up to species level and their populations were counted with the help of a Syracuse counting disc. The roots were examined for gall formation by root-knot nematodes. The number of adult root knot nematode females and larvae were counted. Absolute and relative densities of each nematode species was calculated (Norton 1978)
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