Plant-mycorrhizal fungi associations along an urbanization gradient: implications for tree seedling survival

Abstract

Mycorrhizal fungi symbioses can be critical determining if established seedlings survive or not. Currently, in remnant forests, plants and their fungal symbionts are exposed to varied anthropomorphic effects related to urbanization, however, little is known about their impact on this association. We investigated the relationship between mycorrhizal fungi and tree seedling survival along an urbanization gradient. We planted three species of temperate tree seedlings (Acer rubrum, Carya ovata, and Quercus rubra) in three landscapes: urban, suburban, and rural. We measured the percent of root length colonized by mycorrhizal fungi and monitored survival during their first growing season. We analyzed mycorrhizal colonization as a function of landscape type (urban-rural) and additional variables known to contribute to mycorrhizal colonization (phosphorus, nitrogen, initial plant height). We then analyzed seedling survival as a function of the degree of mycorrhizal fungi colonization associated with the landscape gradient and of additional environmental factors (light and soil moisture). Within a species we found no changes in the levels of mycorrhizal fungi colonization across the urbanization gradient. Each species, however, had markedly different levels of colonization. Survival of A. rubrum was independent of mycorrhizal colonization, while C. ovata and Q. rubra had a significant positive response to increased mycorrhizal fungi. These findings highlight the resilience of mycorrhizal communities across the rural-urban gradient typical of this region, but they also underscore the potential sensitivity of some tree species to lower levels of mycorrhizal fungi colonization.