Abstract
The examples presented in this chapter clearly underline the versatility of hybrid TOF mass spectrometers, and their capabilities with regard to metabolic profiling, structure elucidation and compound identification, using accurate mass determinations and MS/MS fragmentation. The high sensitivity and mass resolution allows the rapid screening of complex plant extracts by DFI, suitable for semi quantitative high throughput (pre)screening. More detailed analysis is possible when MS detection is preceded by applying separation technologies such as LC. Data processing and efficient data handling are becoming more and more the bottleneck in the process, especially when high through put screening is required and the currently available bioinformatics tools are inadequate. Another bottleneck is the low number of available reference compounds needed for definitive identification of differentially accumulating components. Key developments for the near future will therefore have to be made in these areas if true plant metabolomics strategies are to become routine. With better software and more easily mined databases we will be best equipped for the identification of the large numbers of the highly chemically diverse components typically present in complex plant extracts.
Published Version
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