Abstract
Currently, RNAi-based techniques have exhibited considerable potential for pest control, and considerable progress has been made in identifying a large number of potential target genes in the laboratory. However, applying potential target genes effectively in the field to control pests requires further research. At present, transgenic crops expressing dsRNA are widely recognized as the most practical and economical means of applying RNAi-based techniques using laboratory-identified target genes to control pests in the field. Among the three plant-mediated RNAi techniques, plant-mediated RNA interference expressing dsRNA in cytoplasm is most often considered to be an economical and practical RNAi method for pest control, both in the laboratory and inthe field. In this chapter, I describe the procedure for expressing dsRNA in plant cytoplasm for RNAi-based pest control.
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