Abstract
Given the major threat of phytopathogenic bacteria to food production and ecosystem stability worldwide, novel alternatives to conventional chemicals-based agricultural practices are needed to combat these bacteria. The objective of this study is to evaluate the ability of Pseudomonas segetis strain P6, which was isolated from the Salicornia europaea rhizosphere, to act as a potential biocontrol agent given its plant growth-promoting (PGP) and quorum quenching (QQ) activities. Seed biopriming and in vivo assays of tomato plants inoculated with strain P6 resulted in an increase in seedling height and weight. We detected QQ activity, involving enzymatic degradation of signal molecules in quorum sensing communication systems, against a broad range of N-acylhomoserine lactones (AHLs). HPLC-MRM data and phylogenetic analysis indicated that the QQ enzyme was an acylase. The QQ activity of strain P6 reduced soft rot symptoms caused by Dickeya solani, Pectobacterium atrosepticum and P. carotovorum on potato and carrot. In vivo assays showed that the PGP and QQ activities of strain P6 protect tomato plants against Pseudomonas syringae pv. tomato, indicating that strain P6 could have biotechnological applications. To our knowledge, this is the first report to show PGP and QQ activities in an indigenous Pseudomonas strain from Salicornia plants.
Highlights
Given the major threat of phytopathogenic bacteria to food production and ecosystem stability worldwide, novel alternatives to conventional chemicals-based agricultural practices are needed to combat these bacteria
The main objective of our study was to analyze the potential of Pseudomonas segetis strain P6, a novel strain isolated from Salicornia plants, as a biocontrol agent against plant pathogenic bacteria
The analysis showed that strain P6 had 99.93% sequence similarity to Pseudomonas segetis FR1439T
Summary
Given the major threat of phytopathogenic bacteria to food production and ecosystem stability worldwide, novel alternatives to conventional chemicals-based agricultural practices are needed to combat these bacteria. One of the most promising tools used in the agricultural industry is the use of formulations containing plant growth-promoting bacteria (PGPB), known as plant growth-promoting rhizobacteria (PGPR) These are beneficial microorganisms that act as biofertilizers and can fight plant pathogens[7,8]. Given that soil salinization is an upcoming problem in agriculture due to climate change, salt-tolerant PGPB appear to be a suitable approach to deal with the problem of productivity[14,15] Another promising agricultural strategy is the interference of quorum sensing (QS) systems in plant pathogens. AHLs can be degraded or modified by different types of enzymes, including lactonases, acylases and oxidorreductases[28] This strategy has already been reported to reduce the virulence of several plant bacterial pathogens producing promising results[29–31]. The main objective of our study was to analyze the potential of Pseudomonas segetis strain P6, a novel strain isolated from Salicornia plants, as a biocontrol agent against plant pathogenic bacteria
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
