Plant growth regulators mediated mitigation of salt-induced toxicities in mustard (Brassica juncea L.) by modifying the inherent defense system

Abstract

Salt stress is one of the common environmental threats to crop growth, development, and productivity. Plant growth regulators (PGRs) are natural messengers and are known to play pivotal roles at different stages of the growth and development of plants under various environmental conditions. Keeping in mind the importance of PGRs in stress management, a factorial randomized pot experiment was conducted to evaluate the efficiency of three selected PGRs, namely gibberellic acid (GA3), salicylic acid (SA) and triacontanol (Tria) for the amelioration of NaCl stress in mustard. Plants were subjected to four concentrations of NaCl (0, 50, 100 and 150 mM). Two foliar sprays of PGRs (GA3, SA and Tria), each at 5 μM were applied to the foliage of plants using a hand sprayer. The increasing levels of NaCl decreased growth, physio-biochemical, histochemical and yield parameters in a dose-dependent manner while increasing activities of antioxidant enzymes, contents of osmolytes and oxidative stress biomarkers linearly with increasing levels of NaCl. The spray of GA3, SA and Tria under stressed-free and stressed conditions improved the aforesaid attributes while decreasing the generation of stress biomarkers. Of sprayed PGRs, SA proved to be the best for alleviating the adverse effect of NaCl stress. Furthermore, it provides experimental data for its possible biotechnological applications in mustard crops exposed to high concentrations of salinity and possibly to other environmental stresses which have associated oxidative stress.