Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

Nastassja Kimberlly Lima,Pedro Henrique Riboldi Monteiro,Glacy Jaqueline Da Silva,Eloisa Schneider Da Silva,Rayane Monique Sete Da Cruz

doi:10.5296/jas.v8i2.16863

Nastassja Kimberlly Lima, Pedro Henrique Riboldi Monteiro + Show 3 more

Open Access

https://doi.org/10.5296/jas.v8i2.16863

Copy DOI

Abstract

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

Highlights

Acmella oleracea is a species with wide distribution in tropical regions, found in South America, Africa and Asia, belonging to the Asteraceae family and genus Acmella, which is composed of about 30 species
In the northern region of Brazil the species is popularly known as Jambu and is generally cultivated by family farmers (Favoreto and Gilbert, 2010)
Jambu is an annual herbaceous plant that can grow to 40 cm in height in adulthood

Summary

Introduction

Acmella oleracea is a species with wide distribution in tropical regions, found in South America, Africa and Asia, belonging to the Asteraceae family and genus Acmella, which is composed of about 30 species. In the northern region of Brazil the species is popularly known as Jambu and is generally cultivated by family farmers (Favoreto and Gilbert, 2010). The surplus is traded when in excess, at vegetable fairs (Anderberg et al, 2007; Favoreto and Gilbert, 2010; Homma et al, 2011). The economic value attributed to the species for the local population is found in the purpose of use, which is generally applied to regional cuisine, in which it is considered as one of the main ingredients in the preparation of typical dishes, such as “tacacá” and “pato no tucupi”, as well as an ornamental plant in horticulture and for medicinal purposes (Anderberg et al, 2007)

Objectives

Methods

Results

Conclusion