Plant growth regulator eEffect on adventitious roots induction of labisia pumila

Nor’Aishah Hasan,Rusli Ibrahim,Sobri Hussein

doi:10.11113/mjfas.v10n1.70

Nor’Aishah Hasan, Rusli Ibrahim + Show 1 more

Open Access

https://doi.org/10.11113/mjfas.v10n1.70

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Abstract

Labisia pumila or locally known as ‘Kacip Fatimah’ is an herbaceous plant commonly used for dysentery, flatulence, dysmenorrhoea and gonorrhoea. A study was undertaken to investigate the effect of different plant growth regulators at various concentrations on adventitious root induction from the leaf explants of Labisia pumila. Murashige and Skoog (MS) medium supplemented with auxins; indole-acetic acid (IAA), indole-butyric acid (IBA) and naphtaleneacetic acid (NAA) each at the concentrations of 0, 1, 3, 5, and 7 mg/L were evaluated for their effects on adventitious root induction. No adventitious roots formed in the control medium devoid of any PGRs. Among the plant growth regulators (PGRs) used, IBA showed the best adventitious roots formation for all explants, followed by NAA and IAA. Leaf explants cultured on MS medium supplemented with 5 mg/L of IBA showed the best rooting ability. In this treatment, 72.4 ± 9.3 % of rooting, 17.8 ± 9.4 roots per explants and 0.123 ± 0.096g of dry weight were recorded. NAA and IAA showed ability to induce adventitious roots from leaf however the rooting ability was relatively lower that IBA treatment. The highest rooting ability for NAA and IAA was achieved in 7 mg/L and 3 mg/L with (71.2 ±10.9 % and 10.6 ± 3.6%) of rooting, (14.9 ± 0.669 and 1.60 ± 0.179) roots per explants and (0.044 ± 0.002g and 0.014 ± 0.001g) of dry weight, respectively.

Highlights

Older generation were using plants as medicine and the knowledge was passed down orally
In the study of the effect of auxin concentrations on adventitious roots induction from leaf explants, it was found that no root was induced from control medium that did not contain any plant growth regulators (PGRs)
indole-butyric acid (IBA) was the best PGR in the induction of adventitious roots from leaf explants of L. pumila compared to naphtaleneacetic acid (NAA) and indole-acetic acid (IAA)

Summary

INTRODUCTION

Older generation were using plants as medicine and the knowledge was passed down orally. Labisia pumila is one of the valuable medicinal plants and has been used by locals as protective medicine before and after childbirth [2]. Most plants produced a small quantity of secondary metabolites. Tissue culture techniques can be an alternative approach to maintain the sustainability supply of plant materials for producing bioactive compounds continuously under artificially controlled conditions [10]. Adventitious root cultures have been proven to be suitable for continuous production of biochemical and molecular investigation of plant secondary metabolites. Plant growth regulators are the important factor affecting secondary metabolite production. In view of the importance of adventitious roots as well as the limited research on L. pumila, this study was carried out to investigate the effect of plant growth regulators at various concentrations on adventitious roots induction

Culture medium

Data collection

Statistical analysis

RESULTS & DISCUSSION

CONCLUSION