Abstract
The study aimed to isolate and characterize endophytic bacteria associated with Sesbania sesban (L.) Merr. collected in Tan Hung district, Long An province, Vietnam. Bacterial endophytes were isolated and screened capacity of plant growth promotion by using LGI, Burks’ nitrogen free, NBRIP medium. Quantifying bacterial strain’s probability of nitrogen fixation, phosphate solubilization and IAA production was based on colorimetric methods. Isolates with the most potential of nitrogen fixation, phosphate solubilization were identified by using MALDI-TOF Mass Spectrometry. The results were that eighteen bacterial endophytes were isolated from Sesbania sesban (L.) Merr. roots. They had characteristics of promoting plant growth by nitrogen fixation, phosphate solubilization and IAA biosynthesis. DD2 and DD18 with the best capacity of nitrogen fixation and phosphate solubilization were identified as Klesiella pneumonia and Priestia megaterium, respectively. Both were previously reported as plant growth promotion bacteria.
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