Abstract
Streptomyces aureoverticillatus HN6 was isolated in our previous study and effectively controlled banana Fusarium wilt. We explored the role of HN6 in constructing a healthy rhizosphere microflora of banana seedlings. The method of antibiotic resistance was used to determine the colonization ability of HN6. The effect of HN6 on the rhizosphere microbial communities was assessed using culture-dependent and high-throughput sequencing. The effect of HN6 on the infection process of the pathogen was evaluated using a pot experiment and confocal laser scanning microscopy. The results showed that HN6 could prevent pathogen infection; it increased the nutrient content and diversity of the bacterial community in the rhizosphere, promoted plant growth, and decreased the mycotoxin fusaric acid content and abundance of pathogens in the banana rhizosphere. Thus, HN6 decreased the relative abundance of Fusarium species, increased the diversity of fungi, and increased the relative abundance of bacteria in the rhizosphere. HN6 induced the change and reorganization of the microbial community dominated by Fusarium in the rhizosphere of banana seedlings, and it evolved into a community dominated that was not conducive to the occurrence of diseases, shaping the rhizosphere microflora and promoting the growth of banana.
Highlights
The rhizosphere is a unique area that is limited to a few millimeters of soil that is in contact with the root system (Bais et al, 2006)
The results showed that the strain could grow under the condition of 10 μg ml−1 rifampicin, which was better than gentamicin and streptomycin, but it could not grow in the medium containing chlorothalonil (Table 1)
Shen et al (2015) found that the application of Bacillus amyloliquefaciens for 2 years in an area where banana Fusarium wilt occurred could regulate the banana rhizosphere microflora and effectively control Fusarium wilt caused by F. oxysporum and increase the yield
Summary
The rhizosphere is a unique area that is limited to a few millimeters of soil that is in contact with the root system (Bais et al, 2006). Plant roots absorb water and nutrients from the soil, and they affect the adjacent soil through root. Plant Growth-Promoting Rhizobacteria HN6 exudates (Zhang et al, 2017). Plant growth-promoting rhizobacteria (PGPR) provide an effective and environmentally sustainable method to protect crops against soil-borne pathogens (Walley, 1996; Cao et al, 2018). In the process of growth and reproduction, PGPR continuously produce bioactive substances, such as antibiotics, antibacterial proteins, and inhibitory volatile organic compounds (VOCs) (Viaene et al, 2016). Some rhizospheric microorganisms can promote the release of soil nutrients through various metabolic activities and promote plant growth through the production of indole-3-acetic acid (IAA) and siderophore (Wang et al, 2017; Singh et al, 2018; Suleman et al, 2018)
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