Abstract
Co-inoculation of plant growth promoting rhizobacteria (PGPR) with Bradyrhizobiumhas been shown to increase legume nodulation and nitrogen fixation at optimal soil temperatures. Nine rhizobacteria co-inoculated with Bradyrhizobium japonicum532C were tested for their ability to reduce the negative effects of low root zone temperature (RZT) on soybean [ Glycine max(L.) Merr.] nodulation and nitrogen fixation. Three RZTs were tested: 25 (optimal), 17.5 (somewhat inhibitory), and 15°C (very inhibitory). At each temperature some PGPR strains increased the number of nodules formed and the amount of fixed nitrogen when co-inoculated with B. japonicum,but the stimulatory strains varied with temperatures. The strains that were most stimulatory varied among temperatures and were as follows: 15°C, Serratia proteamaculans 1-102; 17.5°C, S. proteamaculans 1-102and Aeromonas hydrophilaP73; 25°C, Serratia liquefaciens2-68.
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