Plant growth promoting microorganisms (PGPM) as an eco-friendly option to mitigate water deficit in soybean (Glycine max L.): Growth, physio-biochemical properties and oil content

Abstract

Drought, as an important challenge in Iran, affects all growth indicators for plants. Application of plant growth promoting microorganisms (PGPM) can reduce the detrimental effects of water deficit on plants. Two separate field experiments were conducted at the Tehran and Hashtrood sites, Iran in 2019 to study the influences of Azotobacter chroococcum (Az) and Piriformospora indica (Pi) or Az + Pi on growth, physio-biochemical properties and oil content of soybean (Glycine max L.) under water deficit conditions. Although water deficit dramatically reduced the plant height, percent vegetation cover and relative water content (RWC), plots treated with Az and Pi exhibited higher performance mentioned traits at both sites. Besides, co-inoculation of Az and Pi increased proline in Tehran (48.85 and 29.24% in leaf and root, respectively) and Hashtrood (46.91 and 48.91% in leaf and root, respectively) under severe water deficit. Accumulation of glycine betaine, soluble sugars and proteins increased for plots which received Az and Pi. Under severe water deficit conditions, the co-inoculation with Az and Pi enhanced the oil content of soybean by 12.87 and 9.37% at Tehran and Hashtrood sites respectively. Application of Az and Pi resulted in reducing the adverse effects of water deficit on oil quality of soybean by increasing the linoleic and linolenic acid in oil. Moreover, inoculation of soybean with Az and Pi can provide drought tolerance by improving ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX) activity. However, co-inoculation with Az and Pi was generally more effective in the alleviation of water deficit detrimental effects than sole inoculation with Az and Pi. Consequently, it can be a good approach for improving tolerance, growth and oil production of soybean under water deficit conditions.