Plant dna barcoding system for forensic application

Abstract

Non-human DNA analysis as well as human DNA analysis is growing importance to investigate the crime. Above all, plant evidence that collected in crime scene can be important key to solve the crime; moving of corpse, route tracking of suspect, identifying of narcotic plant, etc. We optimized the plant DNA barcoding system to analyse from slight amount of plant evidence.We had checked rbcLa, matK and trnL-trnF regions besides psbA-trnH region, and also Dr. MaxⅡ Master Mix(MGmed) for amplifying. The analysis results of 11 species of woody plants and 21 species of herbaceous plants, identified previously, all of woody plants and 19 herbaceous plants were clearly identified its species. Plant DNA barcoding system has been already researched in lots of botanical laboratories. In this study, we used 4 regions to increase discrimination accuracy and domestic taq polymerase for cost-cutting and convenience. As a result, our modified DNA barcoding system is an effective method to analyse slight amount of plant evidence to identify its species and genus.