Plant disease resistance genes: unravelling how they work

Abstract

Resistance (R) genes confer on a plant the ability to defend itself following microbial attack. Each R gene exhibits an extreme specificity of action and is only effective against a microbe that has the corresponding functional avirulence (Avr) gene. This article reviews the strategies and experimental approaches deployed to understand the molecular events underlying the specificity of action of various tomato Cf resistance genes that results in incompatibility to the fungal pathogen Cladosporium fulvum. Topics covered include the clustering of Cf genes, the biology of Cf-dependent incompatibility, the map-based and transposon tagging approaches used to clone the Cf-2 and Cf-9 genes, respectively, identification by mutagenesis of other plant loci required for full Cf-9 mediated resistance, the expression of a functional Avr9 gene in planta and its lethal consequences to Cf-9 containing plants, the physiological and molecular host responses to C. fulvum and AVR elicitor challenges and some genetic approaches to ascertain the crucial components of the defense response. Key words: Cladosporium fulvum, Lycopersicon esculentum, tomato leaf mold, Cf resistance genes, fungal avirulence genes, plant defense responses.