Abstract

Over the past three decades, molecular marker studies reached extraordinary advances, especially for sequencing and bioinformatics techniques. Marker-assisted selection became part of the breeding program routines of important seed companies, in order to accelerate and optimize the cultivar developing processes. Private seed companies increasingly use marker-assisted selection, especially for the species of great importance to the seed market, e.g. corn, soybean, cotton, and sunflower. In the Brazilian public institutions few breeding programs use it efficiently. The possible reasons are: lack of know-how, lack of appropriate laboratories, few validated markers, high cost, and lack of urgency in obtaining cultivars. In this article we analyze the use and the constraints of marker-assisted selection in plant breeding programs of Brazilian public institutes.

Highlights

  • The possibility of using molecular markers in plant breeding was presented in the 80’s by Beckmann and Soller (1986) and Paterson et al (1988)

  • Strong association between genotype and phenotype can be identified with these markers, which is usefull in plant breeding (Ma et al 2010, Mulato et al 2010, Mishra et al 2012, Yamini et al 2013)

  • Molecular markers can be used in many steps of a plant breeding program, e.g. germplasm characterization, pedigree and evolution studies, parental selection for crossing, test for F1 hybrid confirmation, test for genetic purity of seeds, cultivar protection, breeding strategies establishment, linkage map construction, and mapping of genes and quantitative trait loci (QTL) associated with biological processes

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Summary

INTRODUCTION

The possibility of using molecular markers in plant breeding was presented in the 80’s by Beckmann and Soller (1986) and Paterson et al (1988). The advantage of molecular markers over phenotypic data is the possibility to compare genotypes, even if they are sampled in different environment, type of tissue or stage of development Another advantage is the theoretical possibility to detect DNA polymorphisms through the entire genome. Previous review had already indicated the need of improving sampling, genotyping, and analysis techniques, in order to identify reliable markers for plant breeding purposes (Xu and Crouch 2008). These improvements are coming continuously and very fast. In this article we analyze the use and the constraints of marker-assisted selection in plant breeding programs of Brazilian public institutes

MOLECULAR MARKERS IN PLANT BREEDING
Dow Seeds
CONSTRAINTS OF MOLECULAR MARKERS IN PLANT BREEDING
Lack of appropriate laboratories
Few validated markers
High cost
FINAL CONSIDERATIONS
Lack of urgency
Melhoramento de plantas com seleção assistida por marcadores no Brasil

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