Abstract

Ecological monitoring requires sensitive and effective approaches to detecting polluting substances. One such approach involves tracking the behavioral responses of plankton that is initially adapted to normal habitat conditions. Zooplankton such as Daphnia are filter feeders that are highly sensitive to pollution. We describe how a submersible holographic camera with additional attracting lighting (at a wavelength of 532 nm) can be used to track changes of Daphnia response to light for a number of pollutants, including potassium dichromate and crude oil. Our laboratory experiments showed that the sensitivity of this method of bioanalysis is comparable to standard laboratory methods, but has the additional advantage that it can be used in real time in lakes and other water areas, and hence is more versatile than standard methods.

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