Abstract

Theoretical analysis permits the description of absorptive interaction between plane-polarized light and visual pigment in single, side-illuminated, vertebrate photoreceptors. The distribution of light in the aperture cone of the microscope condenser and the distribution of chromophore transition moment in the cylindrical cell are to produce two components of absorption: in the direction of propagation as well as in the plane of polarization. Measured dichroic ratio is precisely interpreted in terms of axial extinction and condenser aperture. The accuracy of the former is analyzed as a function of the latter parameter. Numerical aperture size selection is discussed.

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