Plackett–Burman Combined with Box–Behnken to Optimize the Medium of Fermented Tremella Polysaccharide and Compare the Characteristics before and after Optimization

Xia Ma,Yan He,Chuntao Zhai,Chenglinang Li,Meng Yang,Fatma M El-Demerdash

doi:10.1155/2020/8896454

Abstract

The purpose of this study was to improve the yield of fermented Tremella polysaccharide (FTP) by optimizing the medium and to explore the effect of optimization on the structure. In this paper, the Plackett–Burman design and response surface method were used to optimize the fermentation medium of Tremella spore GT2# for FTP production. The results of the Plackett–Burman design showed that potato extract, glucose, and peptone were the main factors. The Box–Behnken experiment and regression analysis determined that the optimal concentrations of potato extract, glucose, and peptone were 22.01%, 29.38 g/L, and 2.61 g/L, respectively. After optimization, the fermentation period shortened from 5 days to 3 days, and the yield of FTP increased from 10.75 g/L to 12.50 g/L, which was in good agreement with the predicted value (12.36 g/L). Characterization of FTP obtained before and after optimization was carried out, and the results showed that medium optimization had significant effects on the primary structure of FTP, including molecular weight, monosaccharide composition, and proportion. After optimization, the content of acid polysaccharide increased, and the glycoside bonds changed from a-configuration to ß-configuration.

Highlights

Tremella polysaccharide (TP) is a kind of macromolecular substance with multiple biological activities
Single-Factor Experiments. e results (Figure 1) of the single-factor experiment have shown that the optimum range of components of the fermented Tremella polysaccharide (FTP) medium should be as follows: potato extract 20%, glucose 25.0 g/L, peptone 2.5 g/ L, KH2PO4 3.0 g/L, MgSO4·7H2O 1.5 g/L, peptone 2.5 g/L, and Vb1 7.0 mg/L
Glucose (B) has a positive effect on the output of FTP, which means that the higher the glucose content is, the more conducive it is to the production of FTP, and the follow-up optimization should start from the high level of the Plackett–Burman experimental design

Summary

Introduction

Tremella polysaccharide (TP) is a kind of macromolecular substance with multiple biological activities. It is obtained from the fruit body, mycelia, or fermentation broth of Tremella fuciformis. TP of high purity sells for up to 200000 RMB/g. Available TP is mainly obtained from the mature fruit body, but the quality and yield are affected by the environment [10]. High-density fermentation of Tremella fuciformis has many advantages such as easy control of the fermentation conditions, greatly increased yield, reduced production cycle [11], and meeting up with the market demand of fermented Tremella polysaccharides (FTPs)

Methods

Results

Conclusion