Abstract
BackgroundEarly pregnancy loss can be associated with trophoblast insufficiency and coagulation defects. Thrombomodulin is an endothelial-associated anticoagulant protein involved in the control of hemostasis and inflammation at the vascular beds and it's also a cofactor of the protein C anticoagulant pathway.DiscussionWe evaluate the Thrombomodulin expression in placental tissue from spontaneous recurrent miscarriage and voluntary abortion as controls. Thrombomodulin mRNA was determined using real-time quantitative polymerase chain reaction. Reduced expression levels of thrombomodulin were found in recurrent miscarriage group compared to controls (1.82-fold of reduction), that corresponds to a reduction of 45% (from control group Delta CT) of thrombomodulin expression in spontaneous miscarriage group respect the control groups.SummaryWe cannot state at present the exact meaning of a reduced expression of Thrombomodulin in placental tissue. Further studies are needed to elucidate the biological pathway of this important factor in the physiopathology of the trophoblast and in reproductive biology.
Highlights
Pregnancy loss can be associated with trophoblast insufficiency and coagulation defects
Summary: We cannot state at present the exact meaning of a reduced expression of Thrombomodulin in placental tissue
With this background, we performed a prospective casecontrol study measuring the gene expression of thrombomodulin in placental tissues from spontaneous recurrent miscarriage and voluntary abortion by Real-Time quantitative PCR to elucidate the role of this factor on human miscarriage
Summary
We performed a prospective casecontrol study measuring the gene expression of thrombomodulin in placental tissues from spontaneous recurrent miscarriage and voluntary abortion by Real-Time quantitative PCR to elucidate the role of this factor on human miscarriage. In this work we found a reduced expression of Thrombomodulin in placental tissue of women who experienced spontaneous recurrent abortion by RealTime quantitative PCR. This result confirms the observations that loss of function of Thrombomodulin causes early post-implantation embryonic lethality [16], underlying that. Thrombomodulin expression in non-endothelial placental cells is required for a normal function of the early placenta By this hypothesis the absence of Thrombomodulin from blood vessel endothelium may cause excessive activation of the embryonic blood coagulation system [17]. Competing interests The authors declare that they have no competing interests
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