Placental prostacyclin production in normal and toxemic pregnancies

Abstract

Prostacyclin is a potent vasodilator and inhibitor of platelet aggregation. Because toxemia is characterized by increased vasoconstriction frequetly associated with increased platelet aggregation and reduced uteroplacental blood flow, a deficiency in prostacyclin production during pregnancy could contribute to the development of toxemia. Placentally produced prostacyclin could have both local effects on the uteroplacental vasculature and systemic effects because prostacyclin, unlike the other prostaglandins, is not extensively metabolized by the lungs. Fresh human term placentas were obtained immediately after delivery from 12 normal and 12 toxemic (blood pressure ≥14090mm Hg, urinary protein >0.3 gm/24 hours) pregnancies. Tissues (300 mg) were incubated in a sterile manner in 5 ml of Dulbecco's Modifled Eagle's Medium for 48 hours at 37° C with 95% oxygen and 5% carbon dioxide in a metabolic shaker. Samples were collected at 8, 20, 32, and 48 hours and analyzed for prostacyclin by radioimmunoassay of its stable metabolite, 6-keto-prostaglandin F1α. Prostacyclin production was significantly decreased in toxemic placental tissue compared with normal placental tissue(2.72 ± 0.49 versus 7.22 ± 0.44 pg/mg/hr, mean ± SE, p < 0.01). In both normal and toxemic placentas, prostacyclin production was inhibited by indomethacin (5 or 50 μmol/L) and not affected (p > 0.10) by arachidonic acid (5 or 100 μmol/L). Lowering the oxygen concentration from 95% to 20% significently (p < 0.01) decreased prostacyclin production in normal but not toxemic placentas. Prostacyclin production rates in the amnion and chorion were not affected (p > 0.10) by toxemia. The amniotic and chorionic prostacyclin production rates were not different from each other (p > 0.10) and were only one seventh of the normal placental production rate. These data indicate that placental prostacyclin production is decreased in toxemia; therefore, this vasoactive prostaglandin may be involved in the causation and the associated hypertension and coagulation abnormalities of this disorder.