Placental Cryoextract and Renin-Angiotensin-Aldosterone System Blockade Mitigate Renal Failure in Rats

Mykola Repin,Larysa Marchenko,Tetyana Govorukha,Yuliia Chyzh,Oleksandr Brusentsov

doi:10.15407/cryo31.03.223

Abstract

Here, we have studied the impact of administration of rat placental cryoextract (PCE), drug blockade of the renin-angiotensin-aldosterone system (RAAS) with enalapril and spironolactone and their combination on the rat kidney tissue structure and excretory function at different stages of chronic renal failure (CRF) development using the glycerol model. In 3 weeks after glycerol introduction, the animals from all the groups showed low values of glomerular filtration rate, impaired blood flow in renal cortex, tubular epithelial dystrophy, inflammation and edema of interstitium, indicating the onset of CRF development. Tubulo-interstitial nephritis and nephrosclerosis were dominated in untreated rats 16 weeks later. The use of RAAS drug blockade, as well as a comprehensive therapy with RAAS blockers and placental cryoextract stopped the inflammatory processes in renal tissue, restored blood circulation and normalized excretory function, which persisted for up to 16 weeks of observation.

Highlights

We have studied the impact of administration of rat placental cryoextract (PCE), drug blockade of the reninangiotensin-aldosterone system (RAAS) with enalapril and spironolactone and their combination on the rat kidney tissue structure and excretory function at different stages of chronic renal failure (CRF) development using the glycerol model
CRF is accompanied by a decreased glomerular filtration rate (GFR) and microalbuminuria presence
Three weeks after glycerol administration, the group 2 animals had moderate renal excretory dysfunction, as evidenced by oliguria, moderate hypercreatininemia, decreased urinary creatinine, and a significant reduction of GFR down to (0.19 ± 0.06) ml / min (Table)

Summary

Introduction

We have studied the impact of administration of rat placental cryoextract (PCE), drug blockade of the reninangiotensin-aldosterone system (RAAS) with enalapril and spironolactone and their combination on the rat kidney tissue structure and excretory function at different stages of chronic renal failure (CRF) development using the glycerol model. Experimental data suggest using the tissues and cells of fetoplacental complex to be capable of correcting a large number of pathological states and may be one of the new directions for CRF therapy and prevention [4, 5, 8, 9, 25]. It is known, that the mechanisms of CRF pathogenesis, as well as various forms of tubulo-interstitial nephritis mandatory involve the reninangiotensin-aldosterone system (RAAS) [13, 16]. This inevitably entails an increased synthesis and activity of different vasopressors, including the angio-

Methods

Results

Conclusion