Abstract
1. 1. Subcellular fractions of rat liver were assayed for PLA 2 activity. 2. 2. The PLA 2 assay measures the release of [ 3 H]oleic acid from phospholipids, using labeled E. coli as substrate. 3. 3. Nuclear fractions contained PLA 2 activity, which was Ca 2+ dependent and could not be explained from mitochondrial, microsomal or plasma membrane contamination. 4. 4. The V max value of nuclear PLA 2 is 0.30 ± 0.04 pmol oleic acid/min/mg protein; its K m value is 0.86±0.12 μM, similar to that of mitochondrial PLA 2. 5. 5. We conclude that rat liver nuclei contain PLA 2 activity.
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