Abstract
Dihaploid production from elite tetraploid cultivars is key to both traditional and novel breeding approaches that seek to simplify potato genetics. For this purpose, efficient and widely compatible haploid inducers (HIs) are needed. We compared PL-4, a new HI developed at the International Potato Center, to known HIs IvP101 and IvP35. By pollination of elite tetraploid breeding lines, we showed that PL-4 performed significantly better and had a homogeneous response regardless of the genetic background of the pistillate parents, on the most important efficiency traits—number of dihaploids per 100 fruits and haploid induction rate. Moreover, PL-4 exhibited a reduced proportion of hybrid seeds, a convenient trait for efficient screening. In this context, we recommend PL-4 as an enhanced HI for the potato breeding community.
Highlights
The cultivated potato, Solanum tuberosum L. (2n = 4x =48), is a tetraploid crop with polysomic inheritance, and high levels of heterozygosity (Bradshaw 2007)
A total of 9705 pollinations were performed between the CIP elite 4x breeding lines and the haploid inducers (HIs)—IvP35, IvP101 and PL-4, setting a total of 2375 fruits, 21,356 seeds and 1612 dihaploids over the span of three years
We found no significant differences in Fruit set percentage (FSP) among the HIs
Summary
Its polyploid nature complicates genetic studies and can limit genetic gains in breeding. To overcome these problematic aspects, a new breeding. Breeding at the diploid level involves the reduction of ploidy level via the generation of haploid or “dihaploid” (2n = 2x =24) plants from tetraploid varieties or stocks. The hypothetical benefits include a shorter breeding cycle, faster stacking of traits of interest, and more prolific seed production. This could allow for easier variety generation. A large-scale dihaploid generation is a crucial step of this new potato breeding strategy (Lindhout et al 2011)
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