PL - 023 Effects of Oleanolic Acid on Omega-3 and 6 Fatty Acids Metabolism in Rats fed with High Fructose Diet

Abstract

Objective The present study investigated the effect of OA on metabolism of omega 3 and 6 together with genes associated with lipid metabolism in high fructose diet fed Sprague Dawley rats. 
 Methods Real Time PCR qPCR was conducted to evaluate gene expressions levels.
 Gas Chromatography Mass Spectrometry (GS-MS): Lipid metabolism metabolites were assesed.
 Measurement of gene expression using qPCR analysis: Expressions levels of glucose transport and lipid metabolism genes were assessed. 
 Measurements of inflammatory markers concentration in Blood plasma:TNF-α and adiponectin concentrations were determined.
 Results It was found that high fructose diet decreased gene ex pression levels of NRF-1 and GLUT-4 genes, however administration of OA increased expression levels of these genes. Furthermore, adiponectin concentration was lowered in HFD group however this was reversed when HFD was accompanied by OA administration. This increase in concentration of the anti-inflammatory molecule followed the increase in adiponectin receptor, AdipoR1 gene expression in skeletal muscle tissue. The results on lipid metabolism metabolites were consistent the observed molecular events, the anti-inflammatory signaling molecules (EPA and DHA) were five times higher in HF+OA than they were in HFD. High FAS,ACC-2 gene expression levels in HFD group, low 2 expression levels these genes together with increased CPT-1 in OA treated groups coincided with increased levels of arachidonic acid and linoleic acid levels in HFD, threefold increase compared to the control, and decreased levels in OA treated rats. 
 Conclusions Supplementation of diet with oleanolic acid produces DHA and EPA, thus modulating anti-inflammatory response and modulating effects of exercise in reversing diabetes. We provided evidence to effect that diet has a therapeutic potential in managing metabolic syndrome related diseases.