PK-11195: A POTENTIAL DRUG IN LEUKEMIA TREATMENT

Abstract

A ims of the present work include 1. Qualifying anti - cancer activity of PK - 11195 particularly apoptosis inducing effect on Evi1 expressing 3T3 - L1 adip ocyte cell lines. 2. Identifying the role of Ca III as an antioxidant in Evi1 expressing 3T3 - L1 cell lines. 3. Defining the relation of PK - 11195’s pro - apoptotic activity to its reported inhibitory action on mitochondrial ATPase. Cell Viability was measured by MTT assay. Apoptosis and caspase activation was measured by caspase 3/7 Glo assay. Western blotting was used to identify protein expression and oxidative stress was measured by Glutathione Reductase assay , Superoxide dismutase assay and Lipid Peroxidat ion assay. The concluding point that could be made as the results infer is that, by directly inhibiting mitochondrial ATPase PK - 11195 induces oxidative stress hence apoptosis in Evi1 expressing 3T3 - L1adipocytes and this sensitivity of 3T3 - L1 - Evi1 cells is due to the loss of carbonic anhydrase III (Ca III) in those cells.