Abstract
PIXE analysis was successfully applied to the measurement of cellular elements of mammalian cells in relation to the study of hyperthermia. V79 cells were heated at 44.0°C for 30 min or 1 h and the following element releases or uptakes were determined with PIXE measurement of samples obtained after appropriate periods of incubation at 37°C. Linear relationships were confirmed between the produced X-ray yields of P, S, Cl, K and Ca and the cell numbers. The cellular contents P, S, and K decreased promptly after the heat treatment and recovered gradually in the following incubation. Changes in the contents of Cl and Ca did not show abrupt decreases. Curiously, the content of Ca increased after the treatment at 44.0°C for 30 min. The results suggest that various structural modifications were induced in the membrane by the heat treatment.
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