Pivotal role for root cell wall polysaccharides in cultivar-dependent cadmium accumulation in Brassica chinensis L.

Abstract

Polysaccharides are the main components of plant cell walls in which they make an important contribution to cadmium (Cd) fixation. However, knowledge regarding the role of root cell wall polysaccharides in Cd accumulation in low-Cd cultivars is limited. Here, we compared the differences in root cell wall polysaccharides between two cultivars of Brassica chinensis L. (pakchoi) with different Cd accumulation abilities. A hydroponic experiment was conducted using low- (Huajun 2) and high-Cd (Hanlv) pakchoi cultivars. We investigated Cd subcellular distribution and Cd accumulation in cell wall polysaccharides and examined polysaccharide modifications in root cell walls by Fourier transform infrared spectroscopy. A Cd adsorption kinetics experiment was conducted to examine the connection between Cd-induced polysaccharide modifications and Cd fixation by cell walls. Amounts of Cd were significantly higher and more Cd was bound to cell walls in the roots of Huajun 2 than in those of Hanlv. These results indicated that the greater Cd retention capacity of the root cell wall in Huajun 2 accounted for the low Cd accumulation in the shoot. Up to 79.4% and 32.1% of cell-wall-bound Cd was found in the pectin and hemicellulose 1, respectively, and higher amounts of Cd were found in these cell wall components of Huajun 2 than in those of Hanlv. Exposure to Cd significantly increased amounts of pectin and hemicellulose 1 in both pakchoi cultivars, but the pectin levels were significantly higher in Huajun 2 than in Hanlv. Huajun 2 had higher pectin methylesterase (PME) activity and a lower degree of pectin methyl-esterification (DM) than Hanlv, although Cd treatments resulted in increased PME activity and decreased DM in both cultivars. The higher Cd treatment (44.5μM) resulted in enhanced Cd-binding capacity in root cell walls of the two cultivars with higher Cd adsorption levels in the root cell wall of Huajun 2. These results indicate that differences in the amount of cell wall polysaccharide and DM play key roles in establishing the genotypic differences underlying Cd accumulation in pakchoi. These findings conduce to a better understanding of the physiological mechanisms underlying low Cd accumulation in pakchoi and the breeding of new, low-Cd pakchoi cultivars.