Pituitary-adrenocortical responses to the first dyadic encounters in male rhesus monkeys: effect of dominance relationship.

Abstract

Male rhesus monkeys unfamiliar with each other were paired in a cage, and blood samples were collected before and a few hours after pair formation. Adrenocorticotrophic hormone (ACTH) and cortisol levels in each blood sample were measured. Dominant-subordinate status was ascertained through two rank tests, the food competition test and the agonistic behavior test, which were performed immediately after pair formation. As a result, the dominance relationship was determined in seven pairs formed from five animals, and the differences in ACTH and cortisol values between the dominant and subordinate animal in these pairs were compared statistically. The day after the first encounter, a second encounter was conducted in randomly selected pairs of monkeys. In the first encounters, higher levels of both ACTH and cortisol were detected in dominant animals in comparison to subordinate animals. Changing the animal's partner altered the stress responses whenever the animal's dominant-subordinate status changed. The elevated levels of ACTH and cortisol in dominant animals disappeared on the day after the first encounter. In dominant animals, the pituitary-adrenocortical stress response reacts sharply to situational demands, whereas subordinate animals have a weaker response. This acute stress response is different from a chronic stress response. When the subordinate animal cannot escape, its hypothalamic-pituitary-adrenocortical axis appears to be suppressed.