Abstract
An improved method for preparing biologically active polypeptides from the anterior pituitary is described. Two active fractions have been obtained. One fraction accelerates fatty acid synthesis from acetate by liver slices. The other fraction inhibits this synthesis in slices, homogenates and a soluble fraction from liver. This fraction also inhibits the enzymic activity of glyceraldehyde-3-phosphate dehydrogenase and α-glycerophosphate dehydrogenase. It has no effect on the activity of hexokinase, phosphofructokinase, aldolase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, alcohol dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, malic enzyme or aspartate aminotransferase.
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