Pituitary adenylate cyclase-activating polypeptide deficient (PACAP-/-) mice display reduced c-Fos response to forced swim stress

Abstract

Event Abstract Back to Event Pituitary adenylate cyclase-activating polypeptide deficient (PACAP-/-) mice display reduced c-Fos response to forced swim stress B. Gaszner1*, V. Kormos2, D. Reglődi3, T. Kozicz4 and Z. Helyes2 1 Univ. of Pecs, Hungary 2 Pécs University, Department of Pharmacol., Hungary 3 Pécs University, Dept. of Anat., Hungary 4 Radboud University, Dept. of Cell. Animal Physiol., Netherlands PACAP-/- mice show depression-like behavior in forced swim test (FST) and hyperlocomotion in open field (OF). Examination of c-Fos expression could explore which nuclei are involved in the behavioral changes. Here we hypothesized that PACAP-/- mice vs. wildtype counterparts upon OF and FST show altered neuronal activity in stress-related areas. Our results revealed that both locomotor activity in OF and immobility in FST were greater in PACAP-/- mice in accordance with the literature. Counting of c-Fos immunoreactive neurons revealed that stress increased the activity of all examined brain areas. Stressed PACAP-/- mice showed reduced c-Fos response in the oval, anterolateral and anteromedial bed nucleus of the stria terminalis (BNST), dorsal raphe (DR) and Edinger-Westphal nuclei (EW) moreover we found stress x genotype interaction. The c-Fos expression of the periaqueductal gray matter (PAG), paraventricular nucleus (PVN) and basolateral amygdaloid nucleus (BLA) did not drop significantly in stressed PACAP-/- mice but a clear interaction between stress and genotype was observed. The ventral lateral septum (LSv) showed markedly reduced c-Fos expression in transgenic mice without interaction. c-Fos expression in the central and medial amygdala and dorsal lateral septal nucleus were not influenced by PACAP. Based on these we conclude that specific PACAP influence on the BNST, DR, EW, PAG, PVN, BLA and LSv nuclei could play a role in stress-related behavioral changes. Keywords: Behaviour and cognition, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Behaviour and cognition Citation: Gaszner B, Kormos V, Reglődi D, Kozicz T and Helyes Z (2011). Pituitary adenylate cyclase-activating polypeptide deficient (PACAP-/-) mice display reduced c-Fos response to forced swim stress. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00128 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. B. Gaszner, Univ. of Pecs, Pécs, Hungary, balazs.b.gaszner@aok.pte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers B. Gaszner V. Kormos D. Reglődi T. Kozicz Z. Helyes Google B. Gaszner V. Kormos D. Reglődi T. Kozicz Z. Helyes Google Scholar B. Gaszner V. Kormos D. Reglődi T. Kozicz Z. Helyes PubMed B. Gaszner V. Kormos D. Reglődi T. Kozicz Z. Helyes Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.