Pituitary adenylate cyclase-activating polypeptide induces secretion of growth hormone in cattle

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a hypothalamic neuropeptide that stimulates release of growth hormone (GH) from cultured bovine anterior pituitary gland cells, but the role of PACAP on the regulation of in vivo secretion of GH in cattle is not known. To test the hypothesis that PACAP induces secretion of GH in cattle, meal-fed Holstein steers were injected with incremental doses of PACAP (0, 0.1, 0.3, 1, 3, and 10 μg/kg BW) before feeding and concentrations of GH in serum were quantified. Compared with saline, injection of 3 and 10 μg PACAP/kg BW increased peak concentrations of GH in serum from 11.2 ng/ml to 23.7 and 21.8 ng/ml, respectively ( P < 0.01). Peak concentrations of GH in serum were similar in steers injected with 3 or 10 μg PACAP/kg BW. Meal-fed Holstein steers were then injected with 3 μg/PACAP/kg BW either 1 hr before feeding or 1 hr after feeding to determine if PACAP-induced secretion of GH was suppressed after feeding. Feeding suppressed basal concentrations of GH in serum. Injection of PACAP before feeding induced greater peak concentrations of GH in serum (19.2 ± 2.6 vs. 11.7 ± 2.6 ng/ml) and area under the response curve (391 ± 47 vs. 255 ± 52 ng · ml −1 min) than injection of PACAP after feeding, suggesting somatotropes become refractory to PACAP after feeding similar to that observed by us and others with growth hormone-releasing hormone (GHRH). We concluded that PACAP induces secretion of GH and could play a role in regulating endogenous secretion of GH in cattle, perhaps in concert with GHRH.