PITHIA: Protein Interaction Site Prediction Using Multiple Sequence Alignments and Attention.

Abstract

Cellular functions are governed by proteins, and, while some proteins work independently, most work by interacting with other proteins. As a result it is crucially important to know the interaction sites that facilitate the interactions between the proteins. Since the experimental methods are costly and time consuming, it is essential to develop effective computational methods. We present PITHIA, a sequence-based deep learning model for protein interaction site prediction that exploits the combination of multiple sequence alignments and learning attention. We demonstrate that our new model clearly outperforms the state-of-the-art models on a wide range of metrics. In order to provide meaningful comparison, we update existing test datasets with new information regarding interaction site, as well as introduce an additional new testing dataset which resolves the shortcomings of the existing ones.